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Sanitary landfill activities produce leachate that contain of high pollutant wastewater from the solid waste. Anaerobic Fluidized Bed Reactor (AnFBR) is one of technology that able to treat wastewater with high pollutant. This study has purpose to analize the effect of hydraulic retention time (12, 24, 36, and 48 hours ) on the leachate treatment using AnFBR. The leachate collect from TPA Rawa Kucing that has characteristics of COD 5200-5500 mg/L and BOD 2000-2500 mg/L. Seeding and acclimatization is the first method to get the biomass of anaerobic sludge without oxygen supply. During the process, nutrient for the biomass was maintained by giving additional nutrient of glucose 33%, sanitary landfill sludge filtrate 5% and 33% of leachate and 28.3% chlorine-free water at the first formula. The second formula is by mix the glucose 20%, sanitary landfill sludge filtrate 6% and 40% of leachate and 28.3% chlorine-free water, and it tooks 3 months. The results is optimum variations of hydraulic ratention time is 48 hours, to remove BOD, COD, and TSS at 78%, 92% and 22% efficiencies. The degradation rate of the substrate (k) in Anaerobic Fluidized Bed Reactor is 0.73 day-1.