Antioxidant Capacity of Dillenia sp. Leaf Extract Against DPPH (1,1-Diphenyl-2picryl Hidrazil) Radical
Sri Rahayu, Isfi Zahara, Afifah, Krisna Arya Pambudi, Supriyatin
Department of Biology, Faculty of Mathematics and Natural Sciences
Universitas Negeri Jakarta, Indonesia
Abstract
Simpor (Dillenia sp.) is a plant of the Dilleniaceae family. Simpor (Dillenia sp.) has bioactive compound which is flavonoids that act as antioxidant. The purpose of this study was to measure the antioxidant capacity of the 96% etahnolic simpor leaf extract. CRD (Complete Randomized Design) was applied with 3x5 factors. Extraction of leaf samples done by maceration methode using ethanol 96% with variations in maceration time 24 and 48 hours and series consentration of (10%, 15%, and 20%). The antioxidant capacity assay of simpor leaf series concentration (10, 50, 100, 150, dan 200) ppm using the immersion method of the DPPH (1,1-Diphenyl-2picryl Hidrazil) measure absorption at a wavelength ot 517 nm and compared with the powder of ascorbic acid. The result showed that the ethanol extract has flavonoid qualitatively and quantitatively. Furthermore it also has radical scavenging ability against DPPH (1,1-Diphenyl-2picryl Hidrazil).
Keywords: antioxidant capacity, DPPH, leaves simpor, (Dillenia sp.)
Topic: Biology